Testosterone-mediated regulation of mouse renal cytochrome P-450 isoenzymes

Testosterone-mediated regulation of mouse renal cytochrome P-450 isoenzymes.

We have studied the extent to which mouse renal cytochrome P-450 isoenzymes are sexually differentiated, and the factor(s) regulating this dimorphism. Intriguingly, sex differences were not seen in the expression of a single cytochrome P-450 enzyme, but were observed in the expression of all P-450 isoenzymes detectable, encoded by six gene families or sub-families. This effect was mediated by t...

Mouse Liver Testosterone 15 wHydroxylase ( Cytochrome P - 450

Testosterone 15a-hydroxylase (cytochrome P4501a,) was purified from female 129/J mouse liver microsomes based on its specific activities in the eluates from the columns of octylamino-Sepharose 4B, hydroxylapatite, DEAE-Bio-Gel A, and CM52 chromatography. The 150-hydroxylation activity was five times higher in female than in male 129/J mouse liver microsomes. The specific cytochrome P-450 conten...

Cytochrome P-450 isoenzymes involved in fatty acid metabolism.

Regulation of Cytochrome P-450-supported 11P-Hydroxylation

It has been observed that bovine adrenocortical cells in culture fail to synthesize cortisol, while synthesizing deoxycortisol and other precursor steroids. The reason for this loss of activity of 11b-hydroxylase (cytochrome P-450118) was investigated. Treatment of cultures with 30 PM cortisol reduced the half-life of llb-hydroxylase activity from 40 h to 9 h. The concentration of cortisol caus...

Zonation of hepatic cytochrome P-450 expression and regulation.

The CYP genes encode enzymes of the cytochrome P-450 superfamily. Cytochrome P-450 (CYP) enzymes are expressed mainly in the liver and are active in mono-oxygenation and hydroxylation of various xenobiotics, including drugs and alcohols, as well as that of endogenous compounds such as steroids, bile acids, prostaglandins, leukotrienes and biogenic amines. In the liver the CYP enzymes are consti...